Chemistry

Protein separation

Protein separation



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Silver staining of proteins in the gel

Silver staining is one of the most sensitive staining methods for polyacrylamide gels and is particularly suitable when the purity of a protein is to be checked or possible contamination with other proteins is to be detected. Troponin C, calmodulin or some histones not at all.

The chemical reaction is similar to the one used in photography: after fixation, the gel is treated with silver nitrate. Silver nitrate binds (non-stoichiometrically) to proteins and is then reduced.