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Chamber types for development in the DC
Normal chamber (flat-bottom chamber, standard separation chamber)
- Fig.1
- Normal chamber
- most commonly used developing chamber
- for a maximum of two TLC plates
- Lining with solvent-soaked filter paper for saturation (time until saturation: approx. until ), because of the large gas space
Double trough chamber
- Fig 2
- Double trough chamber
- has a threshold on the floor
- less solvent is needed
- Preconditioning of the TLC plate is possible (solvent is in a trough chamber and saturates the gas space. By tilting the chamber, solvent gets into the second trough, development begins.)
Simultaneous separation chamber (synchronous chamber)
- Fig 3
- Simultaneous separation chamber
- has several thresholds on the floor
- less solvent is needed
- Preconditioning of the TLC plate is possible (solvent is in a trough chamber and saturates the gas space. By tilting the chamber, solvent gets into the next trough and development begins.)
Sandwich chamber
- Fig 4
- Sandwich chamber
- ideal conditions for saturated or unsaturated chamber paths with a small gas space
- Either a solvent-soaked second TLC plate or a glass plate is attached to the TLC plate using spacers.
Linear chamber
- Fig.5
- Linear chamber
- Sandwich chamber for horizontal development
- Solvent is filled into troughs
- Glass strips placed on the plate - a capillary suction is created
- Solvent gets on the TLC plate (coating down)
- Development from two sides is possible
Vario chamber
- Fig 6
- Vario chamber
- Sandwich chamber for horizontal development
- several solvent tanks
- suitable for the development of optimal separation conditions (choice of the mobile phase) under the same test conditions
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