Chemistry

Thin layer chromatography (TLC)

Thin layer chromatography (TLC)



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Chamber types for development in the DC

Normal chamber (flat-bottom chamber, standard separation chamber)

Fig.1
Normal chamber
  • most commonly used developing chamber
  • for a maximum of two TLC plates
  • Lining with solvent-soaked filter paper for saturation (time until saturation: approx. 10min until 20min), because of the large gas space

Double trough chamber

Fig 2
Double trough chamber
  • has a threshold on the floor
  • less solvent is needed
  • Preconditioning of the TLC plate is possible (solvent is in a trough chamber and saturates the gas space. By tilting the chamber, solvent gets into the second trough, development begins.)

Simultaneous separation chamber (synchronous chamber)

Fig 3
Simultaneous separation chamber
  • has several thresholds on the floor
  • less solvent is needed
  • Preconditioning of the TLC plate is possible (solvent is in a trough chamber and saturates the gas space. By tilting the chamber, solvent gets into the next trough and development begins.)

Sandwich chamber

Fig 4
Sandwich chamber
  • ideal conditions for saturated or unsaturated chamber paths with a small gas space
  • Either a solvent-soaked second TLC plate or a glass plate is attached to the TLC plate using spacers.

Linear chamber

Fig.5
Linear chamber
  • Sandwich chamber for horizontal development
  • Solvent is filled into troughs
  • Glass strips placed on the plate - a capillary suction is created
  • Solvent gets on the TLC plate (coating down)
  • Development from two sides is possible

Vario chamber

Fig 6
Vario chamber
  • Sandwich chamber for horizontal development
  • several solvent tanks
  • suitable for the development of optimal separation conditions (choice of the mobile phase) under the same test conditions

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